Publications

Cell-spray auto-grafting technology for deep partial- thickness burns: Problems and solutions during clinical implementation

Burns, pages 549-559, October 2017

Cell-spray autografting is an innovative early treatment option for deep partial-thickness burn wounds. As an alternative to non-operative management, cell-spray autografting can achieve rapid wound re-epithelialization, particularly in large wounds. When compared to traditional mesh autografting for deep partial-thickness burn wounds, cell-spray autografting can accomplish re-epithelialization with a much smaller donor site. In this review, we describe the development of a biomedical engineering method for isolation and immediate distribution of autologous, non-cultured, adult epidermis-, and adult dermis-derived stem cells…

Calculations for reproducible autologous skin cell-spray grafting

Burns, pages 1-10, June 2016

Non-cultured, autologous cell-spray grafting is an alternative to mesh grafting for larger partial- and deep partial-thickness burn wounds. The treatment uses a suspension of isolated cells, from a patient’s donor site skin tissue, and cell-spray deposition onto the wound that facilitates re-epithelialization. Existing protocols for therapeutic autologous skin cell isolation and cell-spray grafting have defined the donor site area to treatment area ratio of 1:80, substantially exceeding the coverage of conventional mesh grafting…

Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting

Burns, pages 1-8, February 2016

Partial and deep partial-thickness burn wounds present a difficult diagnosis and prognosis that makes the planning for a conservative treatment versus mesh grafting problematic. A non-invasive treatment strategy avoiding mesh grafting is often chosen by practitioners based on their clinical and empirical evidence. However, a delayed re-epithelialization after conservative treatment may extend the patient’s hospitalization period, increase the risk of infection, and lead to poor functional and aesthetic outcome. Early spray grafting, using non-cultured autologous cells, is under discussion for partial and deep partial-thickness wounds to accelerate the re-epithelialization process, reducing the healing time in the hospital, and minimizing complications…

Latest Test Results Bolster RenovaCare Strategy to Spray Stem Cells on Wounds and Burns for Rapid Healing

May 2016

RenovaCare, Inc., (OTCQB: RCAR) today announced positive spray test results demonstrating that its novel SkinGun™ technology achieves exceptionally-uniform distribution of fluids with 200 times greater coverage than conventional methods. These outcomes are especially promising to scientists developing patented RenovaCare CellMist™ and SkinGun™ technologies* for spraying fluids containing a patient’s own stem cells onto burns and wounds for rapid scar-free healing…

New Study: RenovaCare SkinGun™ Ultra-Gently Sprays Human Stem Cells to Achieve ‘Gold-Standard’ Viability for Skin Regeneration

May 2016

RenovaCare, Inc., (OTCQB: RCAR), a developer of novel medical grade liquid spray devices and patented CellMist™ and SkinGun™ technologies*, today announced favorable outcomes from laboratory studies conducted by Berlin-Brandenburg Center for Regenerative Therapies (BCRT), a translational research center at Charité – Universitätsmedizin Berlin, one of the world’s largest university hospitals…

Effects of wound dressings on cultured primary keratinocytes

Burns, pages 81-90, June 2015

Autologous cell-spray grafting of non-cultured epidermal cells is an innovative approach for the treatment of severe second-degree burns. After treatment, wounds are covered with dressings that are widely used in wound care management; however, little is known about the effects of wound dressings on individually isolated cells. The sprayed cells have to actively attach, spread, proliferate, and migrate in the wound for successful re-epitheliali-zation, during the healing process. It is expected that exposure to wound dressing material might interfere with cell survival, attachment, and expansion…

Method for autologous single skin cell isolation for regenerative cell spray transplantation with non-cultured cells

Int J Artif Organs, pages 271-279, 2011

Background: There is a therapeutic gap for patients with deep partial-thickness wounds (Grade IIb) of moderate size that were initially not treated with split- or mesh grafting to avoid overgrafting, but developed delayed wound healing around two weeks after injury – at which time grafting is typically not indicated anymore. Delayed wound healing is often associated with esthetically unsatisfactory results and sometimes functional problems. An innovative cell isolation method for cell spray transplantation at the point of care, which eliminates cell culture prior to treatment, was implemented for this population of burn patients in our center…

Sprayed Cultured Epithelial Autografts for Deep Dermal Burns of the Face and Neck

Annals of Plastic Surgery • Volume 58, Number 1, January 2007

The objective of this study was the assessment of clinical results after sprayed application of cultured epithelial autograft (CEA) suspensions onto deep dermal burn wounds of the face and neck. Nineteen patients with deep dermal burns of the face and neck were included into a prospective study. The average total body surface area burn was 15.1% (7%– 46%; median: 13%). The average Abbreviated Burn Severity Index (ABSI) was 6.7 points (4 –12 points; median: 7 points). The application of sprayed CEA suspension was performed onto an average body surface area of 2% (0.5–5%; median: 2%). Thirteen patients were recruited for clinical follow-up after an average of 10 months (3–18 months)…